Glycans from Streptococcal Cell Walls

Glycans from Streptococcal Cell Walls

The cell wall of Streptococcus faecalis, strain N, has been found to contain two antigenic glycans, a diheteroglycan of glucose and galactose and a tetraheteroglycan of rhamnose, glucose, galactose, and N-acetylgalactosamine. The present report describes the isolation, purification, and structural and immunochemical characterization of the hitherto unknown diheteroglycan. A tentative structure ...

Hemolytic Streptococcal Cell Walls

INTRODUCTION.................................................................................. 369 STREPTOCOCCAL STRUCTURE................................................................. 369 ISOLATION AND IMMUNOCHEMICAL CHARACTERISTICS OF GROUP CARBOHYDRATES ..... ............ 370 Alethods for Extraction of the Carbohydrates from Cell Walls.................................... 371 Group-Specifi...

Glycans from streptococcal cell walls: structural features of a diheteroglycan isolated from the cell wall of Streptococcus bovis.

Glycans from streptococcal cell walls. The molecular structure of an antigenic diheteroglycan of glucose and galactose from Streptococcus faecalis.

A complete molecular structure for an antigenic glycan isolated from the cell wall of Streptococcus faecalis is being proposed on the basis of analytical data obtained from methylation, acetolysis, and periodate oxidation experiments and on the basis of the nature of the oligosaccharide fragments isolated from partial acid hydrolysates of the glycan. The antigen is a diheteroglycan composed of ...

Biosynthesis of streptococcal cell walls: N-acetyl-D-muramic acid.

Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for acetylglucosamine in providing the C1-C6 moiety of muramic acid. Although rad...